ARHGEF11 antibody (AA 1-50)

Catalog No. ABIN296726

The Rabbit Polyclonal anti-ARHGEF11 antibody is suitable to detect ARHGEF11 in samples from Human, Rat, Mouse, Chicken, Cow, Dog, Horse and Monkey. It has been validated for WB, IHC and IHC (p).

Quick Overview for ARHGEF11 antibody (AA 1-50) (ABIN296726)

Target: ARHGEF11 (rho Guanine Nucleotide Exchange Factor (GEF) 11 (ARHGEF11))

Reactivity: Human, Rat, Mouse, Chicken, Cow, Dog, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARHGEF11 Antibody

Binding Specificity: AA 1-50

Specificity: A portion of amino acids 1-50 of human ARHGEF11.

Purification: Immunoaffinity purified

Immunogen: A portion of amino acids 1-50of human ARHGEF11.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.1 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ARHGEF11

Target: ARHGEF11 (rho Guanine Nucleotide Exchange Factor (GEF) 11 (ARHGEF11))

Alternative Name: ARHGEF11

Background: Name/Gene ID: ARHGEF11

Synonyms: ARHGEF11, GTRAP48, PDZ-RHOGEF, KIAA0380

Gene ID: 9826

UniProt: O15085

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling