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CD99 antibody (Extracellular Domain)

CD99 Reactivity: Human FACS, WB, ELISA Host: Goat Polyclonal unconjugated

Catalog No. ABIN296822

Datasheet as PDF

Target See all CD99 Antibodies

CD99
Binding Specificity
15

6

4

4

3

2

2

2

1

1

1

1

1

1
Extracellular Domain
Reactivity
102

24

9

6

1
Human
Host
73

50

3
Goat
Compatible Secondaries
Clonality
72

54
Polyclonal
Conjugate
49

12

11

8

4

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD99 antibody is un-conjugated
Application
62

52

36

29

21

18

14

13

13

12

6

5

4

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA

Specificity

Selected for its ability to recognize human CD99 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rmCD99.

Purification

Affinity purified

Immunogen

NS0-derived rhCD99 extracellular domain.

Isotype

IgG

Top Product

Discover our top product CD99 Primary Antibody
anti-CD99 (CD99) (AA 1-185) antibody
CD99 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1B10E12 unconjugated

anti-CD99 (CD99) (AA 1-185) antibody
CD99 Reactivity: Human FACS, WB, IHC, ELISA, ICC Host: Mouse Monoclonal 7E11C4 unconjugated

anti-CD99 (CD99) (AA 23-122) antibody
CD99 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3A10 unconjugated

anti-CD99 (CD99) (AA 23-185) antibody
CD99 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-CD99 (CD99) antibody
CD99 Reactivity: Human FACS, CyTOF Host: Mouse Monoclonal 3B2-TA8 unconjugated

anti-CD99 (CD99) (AA 27-161) antibody
CD99 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD99 (CD99) (AA 26-160) antibody
CD99 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD99 (CD99) antibody (PE)
CD99 Reactivity: Human FACS Host: Mouse Monoclonal 3B2-TA8 PE

anti-CD99 (CD99) antibody
CD99 Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 5C8 unconjugated

anti-CD99 (CD99) antibody
CD99 Reactivity: Human FACS, WB, IHC (p), IP Host: Mouse Monoclonal MEM-131 unconjugated

Application Notes

Approved: ELISA, Flo, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody has been validated in whole blood CD3+ T cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by a secondary developing reagent such as donkey anti-goat lgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.2 ng/well.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitute in 500 μL of Sterile PBS.

Concentration

Lot specific

Buffer

Supplied as a powder lyophilized from a solution containing PBS with 5 % trehalose.

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

-20 °C

Storage Comment

Store at -20°C. Avoid freeze-thaw cycles.
Target

CD99

Alternative Name

CD99 (CD99 Products)

Target Type

Viral Protein

Background

Name/Gene ID: CD99

Synonyms: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 Molecule, E2 antigen, HBA71

Gene ID

4267