DKK1 antibody (AA 250-262)

Catalog No. ABIN296913

The Goat Polyclonal anti-DKK1 antibody (ABIN296913) specifically detects DKK1 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Dog, Cow, Horse, Goat, Pig and Monkey samples.

Quick Overview for DKK1 antibody (AA 250-262) (ABIN296913)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Human, Mouse, Dog, Cow, Horse, Goat, Pig, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This DKK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DKK1 Antibody

Binding Specificity: AA 250-262

Specificity: Human DKK1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Bovine, Dog, Goat, Elephant, Panda, Horse, Pig (100%) Gibbon, Rat, Bat, Hamster, Platypus (92%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DHHQASNSSRLHT from the C-terminus of human DKK1 (NP_036374.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Bovine, Dog, Goat, Elephant, Panda, Horse, Pig (100%), Gibbon, Rat, Bat, Hamster, Platypus (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background: Name/Gene ID: DKK1

Synonyms: DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK

Gene ID: 22943

NCBI Accession: NP_036374

UniProt: O94907

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation