RNF216 antibody (AA 350-400)

Catalog No. ABIN297925

This Rabbit Polyclonal antibody specifically detects RNF216 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for RNF216 antibody (AA 350-400) (ABIN297925)

Target: RNF216 (Ring Finger Protein 216 (RNF216))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF216 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RNF216 Antibody

Binding Specificity: AA 350-400

Specificity: KLH-conjugated synthetic peptide corresponding to a portion of human TRIAD3 between amino acids 350-400.

Purification: Protein G purified

Immunogen: Synthetic peptide from human RNF216 / TRIAD3.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for RNF216

Target: RNF216 (Ring Finger Protein 216 (RNF216))

Alternative Name: RNF216 / TRIAD3

Background: Name/Gene ID: RNF216
Family: Zinc Finger

Synonyms: RNF216, TRIAD3, UBCE7IP1, Ring finger protein 216, U7I1, ZIN

Gene ID: 54476

UniProt: Q9NWF9