MARCH4 antibody (AA 300-350)

Catalog No. ABIN298411

This Rabbit Polyclonal antibody specifically detects MARCH4 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Chicken, Horse, Opossum and Zebrafish (Danio rerio).

Quick Overview for MARCH4 antibody (AA 300-350) (ABIN298411)

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Horse, Opossum, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MARCH4 Antibody

Binding Specificity: AA 300-350

Specificity: A portion of amino acids 300-350 of human RNF174.

Purification: Immunoaffinity purified

Immunogen: A portion of amino acids 300-350of human RNF174.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for MARCH4

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Alternative Name: RNF174 / MARCH4

Background: Name/Gene ID: MARCH4

Synonyms: MARCH4, RING finger protein 174, KIAA1399, MARCH-IV, RNF174

Gene ID: 57574

UniProt: Q9P2E8