MMP13 antibody (N-Term)

Catalog No. ABIN301763

The Rabbit Polyclonal anti-MMP13 antibody is suitable to detect MMP13 in samples from Human. It has been validated for IHC and IHC (p).

Quick Overview for MMP13 antibody (N-Term) (ABIN301763)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP13 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MMP13 Antibody

Binding Specificity: N-Term

Specificity: Human MMP13. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Monkey (94%) Marmoset (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from N-terminus of human MMP13. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Monkey (94%), Marmoset (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (17 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 17 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP13

Background: Name/Gene ID: MMP13
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP13, Collagenase 3, Matrix metalloproteinase-13, Interstitial collagenase, MANDP1, MMP-13, CLG3

Gene ID: 4322