CD86 (CD86) antibody (FITC)
| Antigen |
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| Synonyms |
B70, B7-2, LAB72, CD28LG2, MGC34413 |
| Clonality |
Monoclonal (BU63) |
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Host
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Reactivity
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Conjugate
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FITC (38), PE (30), Biotin (21), APC (9), Cy5,PE (7), RPE (6), SPRD (3), PE,Cy7 (2), Alexa Fluor 488 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy5 (1), PE,Cy5 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
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Application
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Flow Cytometry (FACS) (183), Immunoprecipitation (IP) (50), Immunohistochemistry (Frozen Sections) (IHC (fro)) (46), Western Blotting (WB) (30), Functional Studies (Func) (20), ELISA (9), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Blocking Antibody (Inhibition) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN302014 |
| Quantity |
100 tests (Variants) |
| Price |
325.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD86
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Immunogen
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B-lymphoblastoid cell line ARH 77
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Isotype
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IgG1
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Clone
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BU63
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Description
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CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 andof an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed onprofessional antigen-presenting cells and are essential for T cell activation, the bothmolecules can also substitute for each other in this process. The question what are thedifferences in CD80 and CD86 competency has not been fully elucidated yet, there are stillconflicts in results about their respective roles in initiation or sustaining of the T cell immuneresponse.
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Specificity
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The antibody BU63 reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein ofimmunoglobulin supergene family, expressed on professional antigen-presenting cells, suchas dendritic cells, macrophages or activated B lymphocytes.HLDA V, WS Code BP BP072HLDA V, WS Code A A109HLDA VI, WS Code BP 95HLDA VI, WS Code B CD86.9
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Application Notes
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The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
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Buffer
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The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
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Storage
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Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD86 (CD86)", type "Antibodies"
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Hosts
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Mouse (102), Rat (99), Rabbit (3)
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Reactivities
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Mouse (Murine) (104), Human (73), Rat (Rattus) (29), Chimpanzee (2), Monkey (2)
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Applications
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Flow Cytometry (FACS) (183), Immunoprecipitation (IP) (50), Immunohistochemistry (Frozen Sections) (IHC (fro)) (46), Western Blotting (WB) (30), Functional Studies (Func) (20), ELISA (9), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Blocking Antibody (Inhibition) (1), Neutralization (Neut) (1)
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Conjugates
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FITC (38), PE (30), Biotin (21), APC (9), Cy5,PE (7), RPE (6), SPRD (3), PE,Cy7 (2), Alexa Fluor 488 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy5 (1), PE,Cy5 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
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Alternatives 
