Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A) antibody (PE)

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A)

Synonyms: DR4, APO2, CD261, MGC9365, TRAILR1, TRAILR-1, TNFRSF10A

Clonality: Monoclonal (DR-4-02)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN302032

Additional Information

Alternative name: CD261 / TRAIL-R1

Immunogen: Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of theheavy chain of the human IgG1.

Isotype: IgG1

Clone: DR-4-02

Description: TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1.The ligand for this DR4 death receptor has been identified and termed TRAIL, which is amember of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediatesapoptosis and NF kappaB activation in many cells and tissues.Apoptosis, a programmed cell death, is a operating process in normal cellulardifferentiation and development of multicellular organisms. Apoptosis is induced by coupledof certain cytokines (TNF family - TNF, Fas ligand) and their death domain containingreceptors (TNFR1, Fas receptor).

Specificity: The antibody DR-4-02 recognizes TRAIL-R1 (DR4), a human death receptor 4 (468 aminoacids) expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) andin a variety of tumour-derived cell lines.

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 µg/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 0.1 mg/ml

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Restrictions: For Research Use only

Images

Flow Cytometry analysis

Publications

Publications: Vondálová Blanárová, Jelínková, Szöor et al.: "Cisplatin and a potent platinum(IV) complex-mediated enhancement of TRAIL-induced cancer cells killing is associated with modulation of upstream events in the extrinsic apoptotic pathway." in: Carcinogenesis, Vol. 32, Issue 1, pp. 42-51, 2010 (PubMed).