HLA-DR1 (HLA-DR1) antibody (PE)

Antigen: HLA-DR1 (HLA-DR1)

Clonality: Monoclonal (MEM-267)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN302033

Additional Information

Alternative name: HLA-DR1 (empty)

Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Isotype: IgG2b

Clone: MEM-267

Description: HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule isa heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in themembrane. It plays a central role in the immune system by presenting peptides derived fromextracellular proteins. MHC Class II molecules are expressed in antigen presenting cells(APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chaincontains all the polymorphisms specifying the peptide binding specificities. Hundreds ofDRB1 alleles have been described and typing for these polymorphisms is routinely done forbone marrow and kidney transplantation.

Specificity: The antibody MEM-267 specifically binds to the empty but not peptide-loaded form ofHLA-DR1. DR is the isotypes of human MHC Class II molecules expressed onantigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 µg/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 0.1 mg/ml

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Restrictions: For Research Use only

Images

Western blotting analysis of HLA-DR1 in Raji (A) and Jurkat (B) cell lines using MEM-267 antibody.