CD3 (CD3E) antibody (PE)

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (UCHT1)

Host: Mouse

Reactivity: Human, Primate

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN302043

Additional Information

Alternative name: CD3

Immunogen: human thymocytes followed by Sezary T cells

Isotype: IgG1

Clone: UCHT1

Description: CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of Tcells and in regulating the cell surface expression of the TCR complex. T cell activationthrough the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally relatedmembers of the immunoglobulins super family encoded by closely linked genes on humanchromosome 11. The CD3 components have long cytoplasmic tails that associate withcytoplasmic signal transduction molecules. This association is mediated at least in part by adouble tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play arole in TCR-induced growth arrest, cell survival and proliferation.The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% ofthymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells.Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Specificity: The antibody UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on maturehuman T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.HLDA I, WS Code T 3HLDA III, WS Code T 126HLDA III, WS Code T 471HLDA VI, WS Code T 6T-CD3.1

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, 1998 (PubMed).

Torres, Alcover, Zapata et al.: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003 (PubMed).

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, 2004 (PubMed).

Demedts, Brusselle, Vermaelen et al.: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Rieux-Laucat, Hivroz, Lim et al.: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006 (PubMed).