DDDDK Tag antibody

Antigen: DDDDK Tag

Clonality: Monoclonal (F-tag-01)

Host: Mouse

Reactivity: All Species

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN302065

Additional Information

Immunogen: Synthetic peptide: DYKDDDDK conjugated to KLH

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: F-tag-01

Specificity: The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species.The small size of this tag and its high hydrophilicity decrease the probability of interferencewith its expression, proteolytic maturation, antigenicity, localization and function.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Epitope Tags

Restrictions: For Research Use only

Images

Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope.1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)1B - cell nuclei stained with DAPI (blue)1C - merged figures - confirmation of nuclear localization of the fusion protein, cell nuclei stained with DAPI (blue)