CD86 (CD86) antibody
| Antigen |
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| Synonyms |
B70, B7-2, LAB72, CD28LG2, MGC34413 |
| Clonality |
Monoclonal (BU63) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
Flow Cytometry (FACS) (183), Immunoprecipitation (IP) (49), Immunohistochemistry (Frozen Sections) (IHC (fro)) (45), Western Blotting (WB) (29), Functional Studies (Func) (19), ELISA (9), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Blocking Antibody (Inhibition) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN302084 |
| Quantity |
0.1 mg (1 mg/ml) (Variants) |
| Price |
273.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD86
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Immunogen
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B-lymphoblastoid cell line ARH 77
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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BU63
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Description
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CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 andof an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed onprofessional antigen-presenting cells and are essential for T cell activation, the bothmolecules can also substitute for each other in this process. The question what are thedifferences in CD80 and CD86 competency has not been fully elucidated yet, there are stillconflicts in results about their respective roles in initiation or sustaining of the T cell immuneresponse.
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Specificity
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The antibody BU63 reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein ofimmunoglobulin supergene family, expressed on professional antigen-presenting cells, suchas dendritic cells, macrophages or activated B lymphocytes.HLDA V, WS Code BP BP072HLDA V, WS Code A A109HLDA VI, WS Code BP 95HLDA VI, WS Code B CD86.9
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Application Notes
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Functional Application: The antibody BU63 blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4(CD152)-mulg fusion protein to CD86 (B7-2).
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from cell culture supernatant by protein-A affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD86 (CD86)", type "Antibodies"
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Hosts
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Mouse (102), Rat (99), Rabbit (3)
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Reactivities
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Mouse (Murine) (104), Human (73), Rat (Rattus) (29), Chimpanzee (2), Monkey (2)
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Applications
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Flow Cytometry (FACS) (183), Immunoprecipitation (IP) (49), Immunohistochemistry (Frozen Sections) (IHC (fro)) (45), Western Blotting (WB) (29), Functional Studies (Func) (19), ELISA (9), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Blocking Antibody (Inhibition) (1), Neutralization (Neut) (1)
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Conjugates
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FITC (39), PE (30), Biotin (21), APC (9), Cy5,PE (7), RPE (6), SPRD (3), PE,Cy7 (2), Alexa Fluor 488 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy5 (1), PE,Cy5 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
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