Immunoglobulin G (IgG) antibody

Antigen: Immunoglobulin G (IgG)

Synonyms: IgG1, Igh-4, VH7183, EDG2, LPA1, VZG1, GPR26, edg-2, vzg-1, Gpcr26, Mrec1.3, rec.1.3, Edg2, MGC105279

Clonality: Monoclonal (EM-07)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Catalog no.: ABIN302085

Additional Information

Alternative name: IgG (Fc)

Immunogen: Fusion protein of human IgG Fc fragment.

Format: Purified

Isotype: IgG1

Clone: EM-07

Description: Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effectormolecule of the humoral immune response in man. Its concentration in blood plasma ofhealthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasmaimmunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days)and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rateas other immunoglobulins.

Specificity: The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fcfragments.

Application Details

Application Notes: Western Blotting. Application note: The peroxidase conjugate of this antibody is suitable for detection ofhuman IgG Fc fragments by WB. The antibody EM-07 does not crossreact with IgM. ELISA. Application note: The peroxidase conjugate of this antibody is suitable for detection ofhuman IgG Fc fragments by ELISA. The antibody EM-07 does not crossreact with IgM.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Images

IgG Kappa light chain (1), IgG Lamda light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG Kappa light chain (A) and EM-07 antibody against IgG Fc fragment (B).