CD69 (CD69) antibody
| Antigen |
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| Synonyms |
CLEC2C |
| Clonality |
Monoclonal (FN50) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
Flow Cytometry (FACS) (111), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Immunoprecipitation (IP) (17), Western Blotting (WB) (13), Functional Studies (Func) (5), ELISA (4), Immunofluorescence (IF) (4), Immunohistochemistry (IHC) (4), ELISA (Detection) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Enzyme Immunoassay (EIA) (2)
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| Catalog no. |
ABIN302090 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
260.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD69
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Immunogen
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anti-µ-stimulated human B lymphocytes
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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FN50
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Description
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CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliestinducible cell surface molecules acquired during leukocyte activation. This glycoproteinserves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved inlymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage ofHIV infection, and may be also predictive for clinical response to chemoimmunotherapy.
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Specificity
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The antibody FN50 recognizes CD69, an lymphocyte early activation marker.HLDA IV, WS Code A 91
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Application Notes
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Flow Cytometry: Recommended dilution: 5 µg/ml. Immunohistochemistry (frozen sections). Functional ApplicationWhen stimulating peripheral blood T cells, FN50 antibody together with TPA synergisticallyincreases cell volume and RNA/DNA synthesis. Addition of FN50 to stimulated peripheralblood B cells has similar, but weaker effect.
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from cell culture supernatant by protein-A affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells, Immunology
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Restrictions
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For Research Use only
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Publications
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Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).
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Alternatives for antigen "CD69 (CD69)", type "Antibodies"
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Hosts
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Mouse (62), Hamster (52), Rabbit (4)
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Reactivities
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Human (65), Mouse (Murine) (54), Rat (Rattus) (3)
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Applications
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Flow Cytometry (FACS) (111), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Immunoprecipitation (IP) (17), Western Blotting (WB) (13), Functional Studies (Func) (5), ELISA (4), Immunofluorescence (IF) (4), Immunohistochemistry (IHC) (4), ELISA (Detection) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Enzyme Immunoassay (EIA) (2)
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Conjugates
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FITC (19), PE (16), Biotin (11), APC (7), Cy7,PE (5), RPE (4), Cy5,PE (3), Cy5.5 (2), RPE,Cy5 (2), Alexa Fluor 700 (1), PE,Cy5 (1), PE,Cy7 (1), PerCP (1), SPRD (1)
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