Sos antibody

Antigen: Sos

Clonality: Monoclonal (SOS-01)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN302096

Additional Information

Immunogen: Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: SOS-01

Description: The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomainprotein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionallydistinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchangeactivity of Sos is stimulated by allosteric Ras binding. By another (separable) guanineexchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thusintegrates signals that affect both gene expression and cytoskeletal reorganization, theSos-mediated Ras-activation and Rac activation differ in composition and stability of theformed complex.

Specificity: The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDaintracellular protein.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: HeLa human cervix carcinoma cell line. Application note: Reducing conditions.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western Blotting analysis (reducing conditions) of human Sos using anti-Sos (SOS-01).Lane 1: K562 human Caucasian chronic myeloid leukemia cell lineLane 2: RAJI human Burkitt lymphoma cell lineImmunofluorescence staining of Sos in human primary fibroblasts using anti-Sos (SOS-01, green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Immunofluorescence staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01, red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).