AHNAK1 (AHNAK1) antibody

Antigen: AHNAK1 (AHNAK1)

Clonality: Monoclonal (EM-09)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Catalog no.: ABIN302097

Additional Information

Alternative name: AHNAK1

Immunogen: Bacterially expressed fragment of N-terminal domain of human AHNAK1.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: EM-09

Description: AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasmamembrane after an increas of extracellular calcium level or upon proteinkinase C activationand regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 hasbeen implicated in diverse signal transduction proceses affecting cell differentiation andproliferation. In response to calcium-dependent intercellular contacts AHNAK1 formsmultimeric complexes in the plasma membrane, connected with actin and annexin2/S100A10 assemblies and is thus involved in organization of the plasma membranearchitecture. In epithelial cells, AHNAK1 is localized in cytoplasm or ismembrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear,it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

Specificity: The antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor proteinexpressed mainly in epithelial cell, various types of muscle cells and immune cells.

Application Details

Application Notes: Western Blotting - Positive control: HeLa human cervix carcinoma cell line. Immunohistochemistry (frozen sections) -. Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line. Application note: Permeabilization is required. Immunoprecipitation

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunofluorescence staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09, green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).