»
Antibody
»
anti-clathrin Heavy Chain Hc CLTC
clathrin, Heavy Chain (Hc) (CLTC) antibody
| Antigen |
|
| Synonyms |
Hc, CHC, CHC17, CLH-17, CLTCL2, KIAA0034, CLTC, Chc |
| Clonality |
Monoclonal (BF-06) |
|
Host
|
|
|
Reactivity
|
 Alternatives Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine)
|
|
Application
|
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
|
| Catalog no. |
ABIN302098 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
338.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 3 to 5 Business days |
|
Alternative name
|
Clathrin heavy chain
|
|
Format
|
Purified
|
|
Isotype
|
IgM (Matching secondary antibodies)
|
|
Clone
|
BF-06
|
|
Description
|
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results inmembrane invagination. The basic oligomers are composed of three clathrin heavy chain(180 kDa) and three light chain (30 kDa) subunits and the process of polymerization isdynamically regulated by the light chains. Interaction of clathrin with the plasma membraneis mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Anotherproteins, such as endophilin, epsin and amphiphysin are involved in membrane invaginationand clathrin rearrangements. Finally, dynamin functions at the fission stage ofclathrin-mediated endocytosis.
|
|
Specificity
|
The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDaprotein involved in receptor-mediated endocytosis.
|
|
Application Notes
|
Flow Cytometry: Recommended dilution: 2-5 µg/ml. Positive control: human blood leukocytes
|
|
Concentration
|
1 mg/ml
|
|
Purity
|
> 95% (by SDS-PAGE)
|
|
Purification
|
Purified from ascites by precipitation methods and ion exchange chromatography.
|
|
Buffer
|
Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
|
|
Storage
|
Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.
|
|
Research Area
|
Organelles, Transporters
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "clathrin, Heavy Chain (Hc) (CLTC)", type "Antibodies"
|
Hosts
|
Mouse (12), Rabbit (1)
|
|
Reactivities
|
Human (8), Cow (Bovine) (6), Rat (Rattus) (5), Mouse (Murine) (2), Pig (Porcine) (2), Primate (2), Amphibian (1), Hamster (1)
|
|
Applications
|
Western Blotting (WB) (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Enzyme Immunoassay (EIA) (4), Immunofluorescence (IF) (4), Immunoprecipitation (IP) (4), ELISA (2), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
|
