CD79a Molecule, Immunoglobulin-Associated alpha (CD79A) antibody

Antigen: CD79a Molecule, Immunoglobulin-Associated alpha (CD79A)

Synonyms: IGA, MB-1, CD79A, IG-alpha

Clonality: Monoclonal (HM57)

Host: Mouse

Reactivity: Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Horse (Equine), Rabbit, Guinea Pig, Opossum (Didelphimorphia), Chicken

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN302099

Additional Information

Alternative name: CD79a

Immunogen: Synthetic peptide corresponding to amino acids 202-216 of human CD79a

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: HM57

Description: CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They bothare transmembrane proteins with extended cytoplasmic domains containingimmunoreceptor tyrosine activation motives (ITAMs), and together with cell surfaceimmunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are thefirst components of BCR that are expressed developmentally. They appear on pro-B cells inassociation with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-Bcells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin andlater with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a ispresent as an intracellular component. CD79a/b complex interacts with Src-family tyrosinekinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites fordownstream signaling.

Specificity: The antibody HM57 interacts with CD79a (Ig alpha), a 40-45 kDa subunit of B cellantigen-specific receptor (BCR) and its early developmental forms.HLDA V, WS Code BC cB018HLDA VI, WS Code BP 193HLDA VI, WS Code BP 89HLDA VI, WS Code B B103HLDA VI, WS Code B CD79.4

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Intracellular staining of CD79a with anti-CD79a (HM57) PE (gated on leukemic blast cells) in a patient with childhood B-precursor ALL.

Publications

Publications: Faldyna, Samankova, Leva et al.: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, 2007 (PubMed).