Lactate Dehydrogenase C (LDHC) (AA 221-233) antibody

Antigen: Lactate Dehydrogenase C (LDHC)

Synonyms: CT32, LDH3, LDHX, MGC111073, Ldh3, Ldh-3, Ldh-x, Ldhc4, LDHC, MGC127715, LDH-C, LDH-X

Epitope: AA 221-233

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN302133

Additional Information

Alternative name: LDHC (aa 221 - 233)

Gene ID: 3948, 16833 (mouse)

Immunogen: C-DSDKEHWKNVHKQ

Format: Affinity-purified

Description: All names and symbols: LDHC, MGC111073, lactate dehydrogenase C, LDH3, LDHX. Official Symbol: LDHC. RefSeq number(s): NP_002292.1, NP_059144.1

Specificity: Both variants represent identical product (NP_002292.1 and NP_059144.1). Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 30-35kDa band observed in Mouse Testis lysates (calculated MW of 36.6kDa according to human NP_002292.1 and 35.9kDa according to mouse NP_038608.1). Recommended concentration: 0.03-0.1µg/ml. Immunohistochemistry, Strong signal in seminiferous tubules of Mouse Tesits

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN302133 (0.03µg/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN302133 (1µg/ml) staining of paraffin embedded Mouse Testis. Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.

Publications

Publications: Mazzotta, Guerranti, Gozzetti et al.: "Increased serum lactate dehydrogenase isoenzymes in Ph-negative chronic myeloproliferative diseases: a metabolic adaptation?" in: Hematology (Amsterdam, Netherlands), Vol. 11, Issue 4, pp. 239-44, 2006 (PubMed).