Apolipoprotein F (APOF) antibody

Antigen: Apolipoprotein F (APOF)

Synonyms: LTIP, Apo-F, MGC22520, DKFZp781G18150, AI255964, APOF, DKFZp470B1627, apof

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN302173

Additional Information

Alternative name: Apolipoprotein F

Gene ID: 319

Swiss-Prot: NP_001629.1

Immunogen: Peptide with sequence C-KSYDLDPGAGSLE, from the C Terminus of the protein sequence according to NP_001629.1.

Cross-Reactivity: Human

Format: Liquid

Grade: Serum-free

Description: Official Symbol: APOF

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx. 35kDa band observed in Human Liver lysates (calculated MW of 35.4kDa according to NP_001629.1). Recommended concentration: 0.3-1µg/ml. IHC: In paraffin embedded Human Liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cardiovascular, Atherosclerosis, Metabolism

Restrictions: For Research Use only

Images

ABIN302173 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.