PDE8B antibody (Internal Region)

Catalog No. ABIN302525

The Rabbit Polyclonal anti-PDE8B antibody is suitable to detect PDE8B in samples from Human. It has been validated for IHC (p).

Quick Overview for PDE8B antibody (Internal Region) (ABIN302525)

Target: PDE8B (phosphodiesterase 8B (PDE8B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE8B antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDE8B Antibody

Binding Specificity: Internal Region

Specificity: This antibody detects Phosphodiesterase 8B (PDE8B) at internal domain.

Purification: Immunoaffinity chromatography

Immunogen: PDE8B antibody was raised against synthetic peptide from internal domain of human PDE8B.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,3 mg/mL

Buffer: PBS containing 0.09 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for PDE8B

Target: PDE8B (phosphodiesterase 8B (PDE8B))

Alternative Name: PDE8B

Background: Phosphodiesterase 8B, is a high affinity, cAMP-specific phosphodiesterase. Enhanced PDE8B activity has been associated with the phenotypic expression of Gsalpha mutations in human pituitary tumors. PDE8B is absent in normal pituitary but present in GH-secreting adenomas. PDE8B activity is inhibited by dipyridamole, but it is insensitive to rolipram, zaprinast, or IBMX.Synonyms: 5'-cyclic phosphodiesterase 8B, ADSD, Cell proliferation-inducing gene 22 protein, High affinity cAMP-specific and IBMX-insensitive 3', PIG22, PPNAD3, Phosphodiesterase 8B, hsPDE8B

Gene ID: 8622

NCBI Accession: NP_001025022

UniProt: O95263

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process