BACE1 antibody (Internal Region)

Catalog No. ABIN302584

This Rabbit Polyclonal antibody specifically detects BACE1 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for BACE1 antibody (Internal Region) (ABIN302584)

Target: BACE1 (Beta-secretase 1 (BACE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BACE1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BACE1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody deztects Beta-site APP-Cleaving Enzyme 1 (BACE1) at internal domain.

Purification: Immunoaffinity chromatography

Immunogen: BACE1 antibody was raised against synthetic peptide from internal domain of human BACE1.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (2.5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for BACE1

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE1

Background: Bace 1 is responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.Synonyms: ASP2, Aspartyl protease 2, BACE, BACE-1, Beta-secretase 1, Beta-site APP cleaving enzyme 1, Beta-site amyloid precursor protein cleaving enzyme 1, KIAA1149, Memapsin-2, Membrane-associated aspartic protease 2

Gene ID: 23621

NCBI Accession: NP_036236

UniProt: P56817