Cullin 3 antibody

Catalog No. ABIN303084

The Rabbit Polyclonal anti-Cullin 3 antibody (ABIN303084) specifically detects Cullin 3 in WB, IP, IHC (p) and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for Cullin 3 antibody (ABIN303084)

Target: Cullin 3 (CUL3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Cullin 3 Antibody

Specificity: This antibody reacts to amino acids 1-12 of Cul3 (N-terminus) coupled to KLH.

Purification: Delipidation and defibrination

Immunogen: Synthetic peptide

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry on paraffin sections: 1: 500. Western Blot: 1: 500 - 1: 1000. ELISA: 1: 2000 - 1: 10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: Cullin-3

Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.Synonyms: CUL-3, CUL3, KIAA0617

Gene ID: 8452

NCBI Accession: NP_003581

UniProt: Q13618

Pathways: M Phase