MHC Class I H-2Ld antibody (Biotin)

Catalog No. ABIN303513

The Biotin-conjugated Mouse Monoclonal anti-MHC Class I H-2Ld antibody (Clone 30-5-7S) (ABIN303513) specifically detects MHC Class I H-2Ld in FACS. The antibody is reactive with Mouse samples.

Quick Overview for MHC Class I H-2Ld antibody (Biotin) (ABIN303513)

Target: MHC Class I H-2Ld

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Clone: 30-5-7S

Product Details

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Protein G Chromatography.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry (See Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: MHC Class I H-2Ld

Background: The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.Synonyms: H-2 class I histocompatibility antigen, H2-L, L-D alpha chain

Gene ID: 10090

UniProt: P01897