CXCR4 antibody (AA 182-196)

Catalog No. ABIN305765

The Rabbit Polyclonal anti-CXCR4 antibody is suitable to detect CXCR4 in samples from Human and Mouse. It has been validated for WB, IHC and IHC (p).

Quick Overview for CXCR4 antibody (AA 182-196) (ABIN305765)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CXCR4 Antibody

Binding Specificity: AA 182-196

Purification: Ion exchange chromatography

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CXCR4

Background: Name/Gene ID: CXCR4
Subfamily: Chemokine
Family: GPCR

Synonyms: CXCR4, CD184, CD184 antigen, Cmkar4, Cxc chemokine receptor 4, CXCR-4, CXC-R4, D2S201E, FB22, Fusin, HM89, LCR1, NPY3R, NPYRL, NPYY3R, Npyy3, HSY3RR, SDF-1 receptor, WHIM, C-x-c chemokine receptor 4, LESTR, Neuropeptide Y receptor Y3, NPYR

Gene ID: 7852

UniProt: P61073

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events