COL18A1 antibody (C-Term)

Catalog No. ABIN305768

The Rabbit Polyclonal anti-COL18A1 antibody is suitable to detect COL18A1 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for COL18A1 antibody (C-Term) (ABIN305768)

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL18A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-COL18A1 Antibody

Binding Specificity: C-Term

Specificity: Synthetic peptide corresponding to C-terminal residues of mouse Col18a1 (Collagen alpha1(XVIII) chain precursor, contains: Endostatin).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from mouse COL18A1 / Endostatin.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1 μg/mL), IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for COL18A1

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Alternative Name: COL18A1 / Endostatin

Target Type: Chemical

Background: Name/Gene ID: COL18A1
Family: Collagen

Synonyms: COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1(XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1(xviii), Knobloch syndrome, type 1

Gene ID: 80781

UniProt: P39060