OCLN / Occludin (C-Term) antibody

Antigen: OCLN / Occludin (C-Term)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN305888

Additional Information

Swiss-Prot: Q16625

Immunogen: Fusion protein, A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.

Isotype: IgG  (Matching secondary antibodies)

Description: Gene: OCLN / Occludin, Standard Gene Symbol: OCLN, Synonyms: OCLN, BLCPMG, Occludin

Specificity: Recognizes mammalian occludin. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity: Human, mouse, rat and canine. Predicted Reactivities: Dog (Canine),Mammalian,Mouse (Murine),Rat (Rattus).

Application Details

Application Notes: ELISA (0.1 - 1 µg/ml), IF, IHC-P (2.5 µg/ml), IP, WB (1 - 3 µg/ml)

Concentration: 0.25 mg/ml

Purification: Immunoaffinity Chromatography

Buffer: PBS, pH 7.4, 0.1% sodium azide.

Storage: Long term: -20°C, Short term: +4°C

Restrictions: For Research Use only

Images

Human Kidney (formalin-fixed, paraffin-embedded) stained with OCLN antibody ABIN305888 at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen.