GFAP antibody (AA 417-430)

Catalog No. ABIN305893

This Goat Polyclonal antibody specifically detects GFAP in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Rat, Cow, Rabbit, Horse, Monkey and Bat.

Quick Overview for GFAP antibody (AA 417-430) (ABIN305893)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Cow, Rabbit, Horse, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GFAP Antibody

Binding Specificity: AA 417-430

Specificity: Human GFAP.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rat, Bat, Bovine, Elephant, Horse, Rabbit (100%) Mouse, Dog, Pig, Turkey, Chicken (93%) Opossum, Lizard (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DGEVIKESKQEHKD from the C-terminus of human GFAP (NP_002046.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rat, Bat, Bovine, Elephant, Horse, Rabbit (100%), Mouse, Dog, Pig, Turkey, Chicken (93%), Opossum, Lizard (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Name/Gene ID: GFAP

Synonyms: GFAP

Gene ID: 2670

NCBI Accession: NP_002046

UniProt: P14136