CPI-17 antibody (AA 1-147)

Catalog No. ABIN306457

This Mouse Monoclonal antibody specifically detects CPI-17 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for CPI-17 antibody (AA 1-147) (ABIN306457)

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CPI-17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 4H10

Product Details anti-CPI-17 Antibody

Binding Specificity: AA 1-147

Purpose: Human CPI-17/PPP1R14A antibody

Purification: Purified

Immunogen: Recombinant human PPP1R14A (1-147aa) purified from E. coli

Isotype: IgG2a kappa

Application Details

Application Notes: Recommended dilution 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details for CPI-17

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Alternative Name: CPI-17/PPP1R14A

Background: PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.

NCBI Accession: NP_150281