Phosphotyrosine antibody (Peroxidase (POD))

Catalog No. ABIN306704

This Mouse Monoclonal antibody specifically detects Phosphotyrosine in WB, IP, IHC and EIA.

Quick Overview for Phosphotyrosine antibody (Peroxidase (POD)) (ABIN306704)

Target: Phosphotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is conjugated to Peroxidase (POD)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Clone: PY20

Product Details anti-Phosphotyrosine Antibody (Peroxidase (POD))

Purpose: Mouse anti Phosphotyrosine, conjugated with peroxidase

Characteristics: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The peroxidase conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce undesirable background signals.

Purification: Prot A/G

Immunogen: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Isotype: IgG2b

Application Details

Application Notes: Application: A dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunoprecipitation, immunohistochemistry, or immunocytochemistry.

Restrictions: For Research Use only

Handling

Buffer: 60 % glycerol, phosphate buffered saline, and 0.1 % 2-Chloroacetamide

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate