ARHGEF2 antibody (AA 2-447)

Catalog No. ABIN306728

The Sheep Polyclonal anti-ARHGEF2 antibody is suitable to detect ARHGEF2 in samples from Mouse. It has been validated for WB, IF and IP.

Quick Overview for ARHGEF2 antibody (AA 2-447) (ABIN306728)

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This ARHGEF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-ARHGEF2 Antibody

Binding Specificity: AA 2-447

Purpose: Sheep anti Mouse LFC

Cross-Reactivity: Mouse

Characteristics: Lfc is a 118 kDa protein discovered in a screen for oncogenes in NIH 3T3 cells. Lfc is a signaling protein related to Lbc and is part of the Dbl family of proteins. Lfc is a guanine-nucleotide exchange factor (GEF) for the Rho family of small GTPases. Lfc has also been shown to be upstream of the serine-threonine kinase JNK. Lfc has also been shown to mediate the activation of the Rac signaling pathway.

Purification: Ammonium Sulfate Precipitation

Immunogen: Antibody developed using protein corresponding to amino acids 2 to 447 of the Lfc protein fused to GST.

Isotype: IgG

Application Details

Application Notes: Detects Lfc in mouse tissues by immunoprecipitation-Western blot at 4 μg/mL. Detects a 118 kDa band in mouse tissue extracts corresponding to wild-type Lfc. Can also be used in immunofluorescence assays. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for ARHGEF2

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Alternative Name: LFC

Background: Rho guanine nucleotide exchange factor 2, ARHGEF2

UniProt: Q92974

Pathways: Negative Regulation of intrinsic apoptotic Signaling