Kein 7 antibody
Reactivity: Human
IHC (fro), WB
Host: Mouse
Monoclonal
C35
unconjugated
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- Target
- Kein 7
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
- Cross-Reactivity
- Human
- Characteristics
- Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 7 is a basic cytokeratin which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of the ovary, lung and breast but not colon or gastrointestinal tract.
- Purification
- Protein A/G Chromatography
- Immunogen
- Hybridoma produced by the fusion of splenocytes from mice immunized with purified human keratin 7 isolated from HeLa cytoskeleton and mouse myeloma cells.
- Clone
- C35
- Isotype
- IgG1
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- Application Notes
- Detects keratin 7 by Western blot. Detects a 54 kDa band corresponding to keratin 7. Optimal concentration should be evaluated by serial dilutions.
- Restrictions
- For Research Use only
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- Buffer
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- Target
- Kein 7
- UniProt
- P08729
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