Sphingosine 1 Phosphate 3 Receptor (EDG-3) antibody

Catalog No. ABIN306918

The Rabbit Polyclonal anti-Sphingosine 1 Phosphate 3 Receptor (EDG-3) antibody (ABIN306918) specifically detects Sphingosine 1 Phosphate 3 Receptor (EDG-3) in IP and WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for Sphingosine 1 Phosphate 3 Receptor (EDG-3) antibody (ABIN306918)

Target: Sphingosine 1 Phosphate 3 Receptor (EDG-3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Purpose: Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) NT

Cross-Reactivity: Human, Mouse

Characteristics: Endothelial cell differentation gene-3 (EDG3) belongs to a family of G-protein couple receptors whose ligands are lysophospholipids. The ligand for EDG3 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family. They have been implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. The couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG3 is expressed in cardiovascular, leukocyte-containing and other tissues.

Purification: Ammonium Sulfate Precipitation

Immunogen: .

Application Details

Application Notes: Antibody can be used for Western blotting (5-10 μg/mL) and Immunoprecipitation (see reference 4). Optimal concentration should be evaluated by serial dilutions. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: Sphingosine 1 Phosphate 3 Receptor (EDG-3)

Background: Endothelial cell differentiation gene 3 N-terminal, Sphingosine-1-Phosphate Receptor-3 (S1P3)

UniProt: Q99500