TIMP1 antibody

Catalog No. ABIN307121

The Mouse Monoclonal anti-TIMP1 antibody is suitable to detect TIMP1 in samples from Human and Primate. It has been validated for WB, IHC and ELISA.

Quick Overview for TIMP1 antibody (ABIN307121)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 2A5

Product Details anti-TIMP1 Antibody

Purpose: Mouse anti Human Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP-1)

Cross-Reactivity: Human, Macaque

Characteristics: TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases-1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 was found to be identical to EPA (erythroidpotentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart and low levels of TIMP-4 are expressed in liver, brain, lung, thymus and spleen.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human TIMP-1 protein and mouse myeloma Ag8563 cells. Sequence common in monkey

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP-1)

Background: TIMP-1, CLG1, EPA, HCI

UniProt: P01033, Q95KL9