GAL-4, DNA Binding Domain (full length) antibody

Catalog No. ABIN307169

The Mouse Monoclonal anti-GAL-4, DNA Binding Domain antibody is suitable to detect GAL-4, DNA Binding Domain in samples from Saccharomyces cerevisiae. It has been validated for WB and IP.

Quick Overview for GAL-4, DNA Binding Domain (full length) antibody (ABIN307169)

Target: GAL-4, DNA Binding Domain

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 8C-1

Product Details

Binding Specificity: full length

Purpose: Mouse anti Yeast GAL-4 DNA Binding Domain

Cross-Reactivity: Saccharomyces cerevisiae

Characteristics: Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: Recognizes a 106 kDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region. Western blot at 1-10 μg/mL. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: GAL-4, DNA Binding Domain

Alternative Name: GAL-4 DNA Binding Domain

Background: Mouse anti GAL-4 DNA Binding region, gal4, gal 4 yeast