PRKG1 antibody (AA 657-671)

Catalog No. ABIN307178

The Rabbit Polyclonal anti-PRKG1 antibody is suitable to detect PRKG1 in samples from Human, Mouse, Rat, Cow, Pig, Chicken, Dog, Bat, Horse, Monkey and Rabbit. It has been validated for WB, IHC and IHC (p).

Quick Overview for PRKG1 antibody (AA 657-671) (ABIN307178)

Target: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Chicken, Dog, Bat, Horse, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PRKG1 Antibody

Binding Specificity: AA 657-671

Specificity: Recognizes human PKG1 alpha at ~75kD.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Pufferfish (93%) Xenopus, Zebrafish (87%) Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).

Purification: Protein A purified

Immunogen: A 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (residues 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Pufferfish (93%), Xenopus, Zebrafish (87%), Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol, 0.09 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PRKG1

Target: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Alternative Name: PKG / PRKG1

Background: Name/Gene ID: PRKG1
Subfamily: CGMP
Family: Protein Kinase

Synonyms: PRKG1, CGKI, CGMP kinase I, CGMP protein kinase type I, CGK-I, CGK1, PKGI, PKG I, PRKGR1B, PRKG1B, PRKGR1A, PGK, PKG, CGK, CGK 1, CGKI-alpha, CGKI-BETA

Gene ID: 5592

UniProt: Q13976