5-Methylcytidine antibody

Antigen: 5-Methylcytidine

Clonality: Monoclonal (33D3)

Host: Mouse

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN308218

Additional Information

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 33D3

Description: DNA methylation is a DNA modification process, which is involved in the control of gene expression. The 5-Methylcytidine antibody specifically binds the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates.

Specificity: Modified base 5-methylcytidine

Comments:

For research use only

Application Details

Application Notes: MeDIP: Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila. Flow Cyt: Use 10ul of working dilution to label 1000000 cells in 100ul. ICC/IF: Used at a dilution of 1/200 for 16 hrs. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Concentration: 1 mg/ml

Purification: Purified IgG from culture supernatant by Affinity chromatography on Protein A ceramic hyper D F - Filtration on 0.22 µm.

Buffer: Phosphate Buffered Saline 10 mM – NaCl 0.15 M – pH 7.4

Storage: Store at -20 C if preferred. Avoid repeated freezing and thawing as this may denature the antibody. No preservative. Should this product contain a precipitate we recommend microcentrifugation before use.

Research Area: DNA/RNA

Restrictions: For Research Use only

Images

Purity control (SDS PAGE): Polyacrylamide gel 13,5 % under denaturated conditions. Staining: Coomassie Blue. Lane 1: Molecular Weight markers. Lane 2: IgG Isotype Control. Lane 3: ABIN308218 (1 ug).

Publications

Publications: Xing, Shi, Le et al.: "Evidence for transgenerational transmission of epigenetic tumor susceptibility in Drosophila." in: PLoS genetics, Vol. 3, Issue 9, pp. 1598-606, 2007 (PubMed).

Ohta, Sakaide, Wakayama: "Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." in: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, 2009 (PubMed).