MARCH10 antibody (Internal Region)

Catalog No. ABIN308396

The Goat Polyclonal anti-MARCH10 antibody is suitable to detect MARCH10 in samples from Human and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for MARCH10 antibody (Internal Region) (ABIN308396)

Target: MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This MARCH10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MARCH10 Antibody

Binding Specificity: Internal Region

Purpose: MARCH10

Sequence: SRNYPQPRTE E

Specificity: This antibody is expected to recognise both reported isoforms (NP_001094345.1, NP_689811.2), which are identical.

Cross-Reactivity: Cow, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1, NP_689811.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. An additiona

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MARCH10

Target: MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))

Alternative Name: MARCH10

Background: MARCH10, membrane-associated ring finger (C3HC4) 10 , FLJ35757, MARCH-X, RNF190, ring finger protein 190

Gene ID: 162333, 303596

NCBI Accession: NP_001094345, NP_689811