MTHFD1 antibody (Internal Region)

Catalog No. ABIN308408

The Goat Polyclonal anti-MTHFD1 antibody is suitable to detect MTHFD1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for MTHFD1 antibody (Internal Region) (ABIN308408)

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MTHFD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MTHFD1 Antibody

Binding Specificity: Internal Region

Purpose: MTHFD1

Sequence: RGDLNDCFIP CTPK

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RGDLNDCFIPCTPK, from the internal region of the protein sequence according to NP_005947.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 110 kDa band observed in Human Brain (substantia nigra) lysates (calculated MW of 102 kDa according to NP_005947.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MTHFD1

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Alternative Name: MTHFD1

Background: MTHFD1 , methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase , MTHFC, MTHFD, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 1

Gene ID: 4522, 108156, 64300

NCBI Accession: NP_005947

Pathways: Methionine Biosynthetic Process