Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1) antibody

Antigen: Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1)

Synonyms: MTHFC, MTHFD, Dcs, Mthfd, E430024A07Rik, cb325, zgc:55597, MGC137793, Tb07.27M11.970, MTHFD1, DKFZp469M2025, MGC53151, MGC79474

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308408

Additional Information

Alternative name: MTHFD1

Gene ID: 4522, 108156 (mouse), 64300 (rat)

Immunogen: RGDLNDCFIPCTPK

Format: Affinity-purified

Description: All names and symbols: MTHFD1 , methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase , MTHFC, MTHFD, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase, C1-THF synthase, NADP-dependent cyclohydrolase/formyltetrahydrofolate synthetase, cytoplasmic C-1-tetrahydrofolate synthase, methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase 1. Official Symbol: MTHFD1 . RefSeq number(s): NP_005947.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx. 110kDa band observed in Human Brain (substantia nigra) lysates (calculated MW of 102kDa according to NP_005947.2). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Metabolism, Amino Acids

Restrictions: For Research Use only

Images

ABIN308408 (1µg/ml) staining of Human Substantia Nigra lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Dorszewska, Florczak, Rozycka et al.: "Oxidative DNA damage and level of thiols as related to polymorphisms of MTHFR, MTR, MTHFD1 in Alzheimer's and Parkinson's diseases." in: Acta neurobiologiae experimentalis, Vol. 67, Issue 2, pp. 113-29, 2007 (PubMed).