Apolipoprotein L, 1 (APOL1) antibody

Antigen: Apolipoprotein L, 1 (APOL1)

Synonyms: APOL, APO-L, APOL-I, wu:fa95b12, wu:fc30h04, si:rp71-1g18.12

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308434

Additional Information

Alternative name: APOL1

Gene ID: 8542

Immunogen: C-NNNYKILQADQE

Format: Affinity-purified

Description: All names and symbols: APOL1, apolipoprotein L, 1, APO-L, APOL, APOL-I, OTTHUMP00000028705, apolipoprotein L-I, apolipoprotein L1. Official Symbol: APOL1. RefSeq number(s): NP_003652.2, NP_663318.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_003652.2, NP_663318.1). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 45kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 44.0kDa according to NP_003652.2,). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN308434 (1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Perez-Morga, Vanhollebeke, Paturiaux-Hanocq et al.: "Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes." in: Science (New York, N.Y.), Vol. 309, Issue 5733, pp. 469-72, 2005 (PubMed).