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Antibodies
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anti-Lipase, Endothelial (LIPG) antibody
Lipase, Endothelial (LIPG) antibody
| Antigen |
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| Synonyms |
EL, EDL, PRO719, MGC55345, zgc:55345, MGC69106, LIPG, lipe |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (49), Immunofluorescence (IF) (39), Flow Cytometry (FACS) (33), Immunohistochemistry (IHC) (27), ELISA (4), Enzyme Immunoassay (EIA) (4), ELISA (Detection) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN308446 |
| Quantity |
100 ug |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Endothelial lipase
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Gene ID
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9388
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Immunogen
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C-RFNLRTSKDPEHEG
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Format
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Affinity-purified
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Description
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All names and symbols: endothelial lipase, lipase, endothelial, LIPG, EDL, EL, PRO719, endothelial cell-derived lipase, lipoprotein lipase H. Official Symbol: LIPG. RefSeq number(s): NP_006024.1
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Specificity
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Human
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 70kDa band observed in Human Liver lysates (calculated MW of 56.8kDa according to NP_006024.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gauster et al , J Clin Endocrinol Metab. 2007 Jun,92(6):2256-63. Epub 2007 Mar 13., PMID: 17356047). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Jin, Wang, Millar et al.: "Hepatic proprotein convertases modulate HDL metabolism." in: Cell metabolism, Vol. 6, Issue 2, pp. 129-36, 2007 (PubMed).
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Alternatives for antigen "Lipase, Endothelial (LIPG)", type "Antibodies"
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Hosts
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Rabbit (33), Mouse (32), Goat (3)
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Reactivities
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Human (60), Mouse (Murine) (23), Rat (Rattus) (20), Pig (Porcine) (3), Cow (Bovine) (2), Sheep (Ovine) (2), Chicken (1), Dog (Canine) (1)
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Applications
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Western Blotting (WB) (49), Immunofluorescence (IF) (39), Flow Cytometry (FACS) (33), Immunohistochemistry (IHC) (27), ELISA (4), Enzyme Immunoassay (EIA) (4), ELISA (Detection) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Alternatives 
