Parp3 (PARP3) antibody

Antigen: Parp3 (PARP3)

Synonyms: Adprt3, PARP-3, Adprtl3, AW990611, pADPRT-3, A930002C11Rik, IRT1, ADPRT3, ADPRTL2, ADPRTL3, PADPRT-3

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN308468

Additional Information

Alternative name: PARP3

Gene ID: 10039

Immunogen: YEDYNCTLNQTNIEN

Format: Affinity-purified

Description: All names and symbols: PARP3, poly (ADP-ribose) polymerase family, member 3, ADPRT3, ADPRTL2, ADPRTL3, IRT1, hPARP-3, pADPRT-3, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase)-like 2, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase)-like 3, NAD+ ADP-ribosyltransferase 3, poly(ADP-ribose) polymerase 3, poly(ADP-ribose) synthetase-3. Official Symbol: PARP3. RefSeq number(s): NP_001003931.2, NP_001003935.2

Specificity: This antibody is expected to recognize both reported isoforms (NP_001003931.2, NP_001003935.2). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Preliminary experiments in lysates of cell lines Daudi, HeLa and HepG2 gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Publications: Rouleau, McDonald, Gagne et al.: "PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery." in: Journal of cellular biochemistry, Vol. 100, Issue 2, pp. 385-401, 2007 (PubMed).