RuvB-Like 1 (E. Coli) (RUVBL1) antibody

Antigen: RuvB-Like 1 (E. Coli) (RUVBL1)

Synonyms: RVB1, TIH1, ECP54, TIP49, INO80H, NMP238, PONTIN, TIP49A, Pontin52, Tip49a, 2510009G06Rik, pontin, zpontin, wu:fd18e04, tip49, xpontin, pontin52, RUVBL1, rvb1, ecp54, nmp238, tip49a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Zebrafish, Xenopus laevis, Dog (Canine), Chicken

Application: Western Blotting (WB)

Catalog no.: ABIN309654

Additional Information

Alternative name: RUVBL1 / TIP49A

Gene ID: RUVBL1

UniProt: Q9Y265

Immunogen: The immunogen for anti-RUVBL1 antibody: synthetic peptide directed towards the N terminal of human RUVBL1

Sequence: KIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVG QENAREACGV

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-RUVBL1 antibody concentration is 1 mg/ml.

Description: RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is the component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.

Characteristics: Antigen Size: 456 amino acids

Molecular Weight: 50kDa

Application Details

Application Notes: WB Suggested Anti-RUVBL1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human heart.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-RUVBL1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human heart

Publications

Product: Jha, Shibata, Dutta: "Human Rvb1/Tip49 is required for the histone acetyltransferase activity of Tip60/NuA4 and for the downregulation of phosphorylation on H2AX after DNA damage." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2690-700, 2008 (PubMed).