CPSF1 antibody (Middle Region)

Catalog No. ABIN310073

Product Details anti-CPSF1 Antibody

Target: CPSF1 (Cleavage and Polyadenylation Specific Factor 1, 160kDa (CPSF1))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GCYDMWTVIA PVRKEEEDNP KGEGTEQEPS TTPEADDDGR RHGFLILSRE

Predicted Reactivity: Horse: 79%, Human: 100%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against CPSF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CPSF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1443 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CPSF1

Target: CPSF1 (Cleavage and Polyadenylation Specific Factor 1, 160kDa (CPSF1))

Alternative Name: CPSF1 (CPSF1 Products)

Background: CPSF1 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
Alias Symbols: CPSF160, HSU37012, P/cl.18
Protein Interaction Partner: REL, RBBP6, UBC, STAU1, LGR4, WWOX, RPA3, RPA2, RPA1, EED, EZH2, NEDD4, TARDBP, ICK, CLK2, HDAC6, NPM1, IL7R, HMOX1, TUT1, FIP1L1, RNPC3, WDR33, CPSF2, MED8, CPSF3, GTF3C3, POLR2A, COPS6, CUL3, SIRT7, SMARCAD1, CSTF2T, PAPOLA, CSTF3, GLIS2, RRAGA, TBP, TA
Protein Size: 1443

Molecular Weight: 161 kDa

Gene ID: 29894

NCBI Accession: NM_013291, NP_037423

UniProt: Q10570