ALG1 antibody (N-Term)

Catalog No. ABIN311183

The Rabbit Polyclonal anti-ALG1 antibody is suitable to detect ALG1 in samples from Human, Mouse, Rat, Rabbit, Cow and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for ALG1 antibody (N-Term) (ABIN311183)

Target: ALG1 (Chitobiosyldiphosphodolichol beta-Mannosyltransferase (ALG1))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALG1 Antibody

Binding Specificity: N-Term

Purpose: ALG1 Antibody - N-terminal region

Sequence: VVLGDVGRSP RMQYHALSLA MHGFSVTLLG FCNSKPHDEL LQNNRIQIVG

Predicted Reactivity: Cow: 79%, Human: 100%, Mouse: 85%, Rabbit: 79%, Rat: 79%, Yeast: 100%

Characteristics: This is a rabbit polyclonal antibody against ALG1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ALG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP49445-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ALG1

Target: ALG1 (Chitobiosyldiphosphodolichol beta-Mannosyltransferase (ALG1))

Alternative Name: ALG1

Background:

Background Information: ALG1 catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. Defects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K).The biosynthesis of lipid-linked oligosaccharides is highly conserved among eukaryotes and is catalyzed by 14 glycosyltransferases in an ordered stepwise manner. Mannosyltransferase I (MT I) catalyzes the first mannosylation step in this process.[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-421 BM767933.1 1-421 422-1179 AY359073.1 416-1173 1180-1444 CA455103.1 259-523 1445-1939 CD366777.1 17-511 c 1940-2122 BC031095.1 1931-2113 2123-2149 BQ002699.1 1-27 c

Gene Name: Asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)

Alternative Symbols: HMT1, MT-1, CDG1K, HMAT1, HMT-1, Mat-1, hMat-1

Protein Name: Chitobiosyldiphosphodolichol beta-mannosyltransferase

Molecular Weight: 52kDa

Gene ID: 56052

NCBI Accession: NP_061982

UniProt: Q9BT22