COPA antibody (N-Term)

Catalog No. ABIN311471

The Rabbit Polyclonal anti-COPA antibody has been validated for WB. It is suitable to detect COPA in samples from Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig, Rabbit, Cow, Goat and Saccharomyces cerevisiae. There are 2+ publications available.

Quick Overview for COPA antibody (N-Term) (ABIN311471)

Target: COPA (Coatomer Protein Complex, Subunit alpha (COPA))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig, Rabbit, Cow, Goat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COPA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-COPA Antibody

Binding Specificity: N-Term

Sequence: PWILTSLHNG VIQLWDYRMC TLIDKFDEHD GPVRGIDFHK QQPLFVSGGD

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against COPA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human COPA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1224 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-COPA antibody (ABIN311471)

Li, Custer, Gilson, Hao, Beattie, Androphy: "α-COP binding to the survival motor neuron protein SMN is required for neuronal process outgrowth." in: Human molecular genetics, Vol. 24, Issue 25, pp. 7295-307, (2015) (PubMed).

Ting, Wen, Liu, Hsieh-Li, Li, Lin-Chao: "The spinal muscular atrophy disease protein SMN is linked to the Golgi network." in: PLoS ONE, Vol. 7, Issue 12, pp. e51826, (2013) (PubMed).

Target Details for COPA

Target: COPA (Coatomer Protein Complex, Subunit alpha (COPA))

Alternative Name: COPA

Background: In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone.In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms.
Alias Symbols: FLJ26320, HEP-COP
Protein Interaction Partner: UBC, SUMO2, SMN1, STAU1, NUDCD1, SUMO1, NEDD8, RPA3, RPA2, RPA1, SUZ12, RNF2, COPZ1, DHX38, COPB2, CCT3, TCP1, SEPT2, ARCN1, UBD, VCAM1, ITGA4, COPB1, COPG2, COPG1, COPE, SEPT9, SEC23A, SEC24C, UBXN6, FBXO6, LMAN1, SACM1L, DDX20, TMED10, GEMIN2, COPA, CDK
Protein Size: 1224

Molecular Weight: 135 kDa

Gene ID: 1314

NCBI Accession: NM_004371, NP_004362

UniProt: P53621

Pathways: Hormone Activity