Coatomer Protein Complex, Subunit beta 1 (COPB1) antibody

Antigen: Coatomer Protein Complex, Subunit beta 1 (COPB1)

Synonyms: COPB, FLJ10341, FLJ46444, FLJ57957, DKFZp761K102, beta'-COP, 2610019B04Rik, Rack2, MGC92482, zgc:92482, id:ibd5034, wu:fc66c05, MGC80934, COPB1, MGC139703, DKFZp459K154

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Xenopus laevis, Chicken, Zebrafish, Fruit Fly (Drosophila melanogaster)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN311506

Additional Information

Alternative name: Coatomer subunit beta (COPB)

Gene ID: COPB1

UniProt: P53618

Immunogen: The immunogen for anti-COPB1 antibody: synthetic peptide directed towards the middle region of human COPB1

Sequence: QLALDLVSSRNVEELVIVLKKEVIKTNNVSEHEDTDKYRQ LLVRTLHSCS

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-COPB1 antibody concentration is 1 mg/ml.

Description: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins, the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.

Characteristics: Antigen Size: 953 amino acids

Molecular Weight: 107kDa

Application Details

Application Notes: WB Suggested Anti-COPB1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Liver.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-COPB1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Liver

Publications

Product: Goryachev, Pokhilko: "Computational model explains high activity and rapid cycling of Rho GTPases within protein complexes." in: PLoS computational biology, Vol. 2, Issue 12, pp. e172, 2007 (PubMed).