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Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F) antibody

Antigen

Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F)

Synonyms AI851109, 2510010F15Rik, NCE2, MGC18120, MGC105540, RGD1307608, ube2f, MGC78786
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Xenopus laevis

Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
1 reference available
Catalog no. ABIN311523
Quantity 50 µg  (Variants)
Price 317.90 $   Plus shipping costs $45.00
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Additional Information

Alternative name NCE2
Gene ID UBE2F
UniProt Q969M7
Immunogen The immunogen for anti-UBE2F antibody: synthetic peptide directed towards the N terminal of human UBE2F
Sequence MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKE VAELEANLPC
Cross-Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
Format Lyophilized
Reconstitution Add 50 µl of distilled water. Final anti-UBE2F antibody concentration is 1 mg/ml.
Description UBE2F belongs to the ubiquitin-conjugating enzyme family. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
Characteristics Antigen Size: 185 amino acids
Molecular Weight 21kDa

Application Details

Application Notes WB Suggested Anti-UBE2F Antibody Titration: 0.2-1 ug/ml
Positive Control: Hela cell lysate.
Purification Affinity Purified
Buffer PBS
Storage (Long Term) For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Storage (Short Term) Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
Research Area Proteolysis / Ubiquitin
Restrictions For Research Use only

Publications

Product Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Alternatives

Alternatives for antigen "Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F)", type "Antibodies"
Hosts Rabbit (37), Goat (8)
Reactivities Human (40), Mouse (Murine) (23), Rat (Rattus) (21), Cow (Bovine) (20), Chicken (18), Dog (Canine) (18), Horse (Equine) (18), Pig (Porcine) (18), Rabbit (18)
Applications Western Blotting (WB) (27), ELISA (24), Immunofluorescence (IF) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (IHC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes N-Term (9), C-Term (5), C-Term, Glu170 (1), Internal Region (1)