Macrophage/Monocyte antibody

Catalog No. ABIN317438

The Mouse Monoclonal anti-Macrophage/Monocyte antibody is suitable to detect Macrophage/Monocyte in samples from Guinea Pig. It has been validated for FACS and IHC (fro).

Quick Overview for Macrophage/Monocyte antibody (ABIN317438)

Target: Macrophage/Monocyte

Reactivity: Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MR-1

Product Details

Purification: Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum

Immunogen: Guinea Pig lymph node stroma

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1/10. Flow cytometry: use 10 μL neat - 1: 10 diluted antibody to label 10^6 cells in 100 μL,membrane permeabilisation is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Should this product contain a precipitate we recommendmicrocentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Macrophage/Monocyte

Alternative Name: Macrophages / Monocytes

Background: Monocyte and macrophage are white blood cells that roam the body tissues engulfing foreign organisms. A monocyte is a leukocyte, part of the human body's immune system that protects against blood-borne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues. Monocytes are usually identified in stained smears by their large bi-lobed nucleus. Macrophages are cells within the tissues that originate from specific white blood cells called monocytes. Monocytes and macrophages are phagocytes, acting in both nonspecific defense (or innate immunity) as well as specific defense (or cell-mediated immunity) of vertebrate animals. Their role is to phagocytize (engulf and then digest) cellular debris and pathogens either as stationary or mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen.Synonyms: Macrophage marker, Monocyte marker